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Introduction Most books on Protein NMR focus on theoretical aspects and pulse sequences with only little space devoted to resonance assignment and structure calculations. At the same time many softwar...

Triple Resonance Backbone Assignment Standard triple resonance backbone assignment of proteins is based on the CBCANNH and CBCA(CO)NNH spectra. The idea is that the CBCANNH correlates each NH group wi...

Chemical Shift Mapping Chemical shifts are very sensitive to the electronic environment of a nucleus. Perturbations in the chemical shift can be caused not only by a change in the covalent molecular s...

References: Biochemistry D. Marion, L.E. Kay, S.W. Sparks, D.A. Torchia and A. Bax (1989) J. Am. Chem. Soc. E.R.P. Zuiderweg and S.W. Fesik (1989) Biochemistry Minimum labelling: Dimensions: 3 To ...